BW5147.G.1.4.OUAR.1

88100507, mouse blood, Lymphoblast

• UNSPSC Code: 41106514

Properties

• Product Name: BW5147.G.1.4.OUAR.1, 88100507, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88100507, mouse blood, Lymphoblast
• biological source: mouse blood
• packaging: tube of 5 μg 88100507-DNA-5UG; pkg of vial of cells 88100507-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse AKR/J T cell lymphoma

Cell Line Description

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100βM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Application

Resistance and hybridisation studies

Culture Medium

DMEM + 2mM Glutamine + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.