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Sigma-Aldrich

iTRAQ® Reagents Multiplex Kit

Synonym(s):

isobaric tags for relative and absolute quantitation 

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.24

shipped in

dry ice

storage temp.

−20°C

General description

Contains iTRAQ® reagents 114, 115, 116, 117, the appropriate buffers and reagents for ten 2-plex, six 3-plex, or five 4-plex assays. 10-PK trypsin is sold separately. Each individual reagent is capable of labeling up to 100 μg of protein.

Application

The iTRAQ® Reagents Multiplex Kit provides iTRAQ® reagents and protocol to label peptides after digestion. This widely used generic labeling kit can be used for virtually any workflow.
iTRAQ® Reagents Multiplex Kit has been used for the labeling of peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.

Analysis Note

To view the general Protocol for iTRAQ Reagents, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

The Certificate of Analysis (CofA) for your specific lot is accessible by entering the product number and lot number at this CofA access link.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

35.6 °F - closed cup

Flash Point(C)

2.0 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.
Zeiner CA, et al.
PLoS ONE, 11, e0157844-e0157844 (2016)
Debra Rossouw et al.
Applied and environmental microbiology, 76(12), 3911-3923 (2010-04-27)
The geno- and phenotypic diversity of commercial Saccharomyces cerevisiae wine yeast strains provides an opportunity to apply the system-wide approaches that are reasonably well established for laboratory strains to generate insight into the functioning of complex cellular networks in industrial
Xiuyan Huang et al.
Journal of proteome research, 9(12), 6759-6773 (2010-10-20)
Regulatory T cells (Treg) induce robust neuroprotection in murine models of neuroAIDS, in part, through eliciting anti-inflammatory responses for HIV-1-infected brain mononuclear phagocytes (MP; macrophage and microglia). Herein, using both murine and human primary cell cultures in proteomic and cell
Ken H Loh et al.
Cell, 166(5), 1295-1307 (2016-08-28)
Cellular compartments that cannot be biochemically isolated are challenging to characterize. Here we demonstrate the proteomic characterization of the synaptic clefts that exist at both excitatory and inhibitory synapses. Normal brain function relies on the careful balance of these opposing
Penggao Dai et al.
Journal of immunology (Baltimore, Md. : 1950), 182(6), 3450-3460 (2009-03-07)
Emerging evidences suggest TLR-mediated signaling is tightly regulated by a specific chain of intracellular protein-protein interactions, some of which are yet to be identified. Previously we utilized a dual-tagging quantitative proteomics approach to uncover MyD88 interactions in LPS-stimulated cells and

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