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HKG6PDH-RO

Roche

Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)

from yeast/Leuconostoc, overproducer

Synonym(s):

G6P-DH, Glucose-6-Phosphate DehydrogenaseHK, Hexokinase

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About This Item

UNSPSC Code:
12352204

biological source

yeast

Quality Level

100

form

suspension

mol wt

dimer 110,000 Da

packaging

pkg of 10 mL (10737275001 [30 mg])
pkg of 5 mL (10127825001 [15 mg])

manufacturer/tradename

Roche

concentration

3.21 mg/mL

technique(s)

activity assay: suitable

color

white

pH

5.5-6.5

solubility

water: miscible

suitability

suitable for UV spectrophotometry and general use

application(s)

life science and biopharma

storage temp.

2-8°C

General description

Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.

Application

Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.

Components

EC 2.7.1.1 & 1.1.1.49

Unit Definition

One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.

Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.

Other Notes

For life science research only. Not for use in diagnostic prodcedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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