SF001
ESGRO Complete PLUS Clonal Grade Medium, 100ml
to enhance viability of mouse embryonic stem cells
Synonym(s):
Mouse ES Cell Media
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.81
Recommended Products
Quality Level
100
300
form
liquid
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
General description
ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency.
Application
Suitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth.
Media can be used on feeder dependent/independent ES cell lines.
Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Media can be used on feeder dependent/independent ES cell lines.
Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Components
1. ESGRO Complete Clonal Grade Medium: (Catalogue No. SF001-C) One (1) 100 mL bottle containing original clonal grade medium. Complete formulation containing self-renewal factors, no need to add LIF. pH 7.1-7.3
2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 µL vial containing selective GSK3β inhibitor.
2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 µL vial containing selective GSK3β inhibitor.
Quality
QC PROTOCOL:
Low density plating assay:
1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
2. Change medium every 2-4 days.
3. Monitor for clonal growth with alkaline phosphatase stain.
Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation.
Low density plating assay:
1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
2. Change medium every 2-4 days.
3. Monitor for clonal growth with alkaline phosphatase stain.
Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation.
Linkage
Replaces: SF001-100
Storage and Stability
• Upon receipt, store clonal grade medium and supplement in the dark at -20°C. Refer to lot expiration date on labels.
• Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze.
• Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months.
• After supplement is added to clonal grade medium, store in the dark at 4°C for up to
two weeks. Do not refreeze.
• Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze.
• Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months.
• After supplement is added to clonal grade medium, store in the dark at 4°C for up to
two weeks. Do not refreeze.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Lact. - Repr. 1A
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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