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MAB8293

Sigma-Aldrich

Anti-Parvovirus B19 Antibody, aa 328-344 of VP2 capsid protein, clone R92F6

clone R92F6, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

R92F6, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

shipped in

wet ice

Specificity

Recognizes Human parvovirus B19. Recognizes an epitope common to VP1 and VP2 structural proteins of B19. In B19 infected tissues, MAB8293 staining is localized predominantly in the nucleus though cytoplasmic staining may also be observed.

Immunogen

Epitope: a.a. 328-344 of VP2 capsid protein
Native parvovirus B19 purified from human plasma.

Application

Anti-Parvovirus B19 Antibody, aa 328-344 of VP2 capsid protein, clone R92F6 is an antibody against Parvovirus B19 for use in ELISA, IF, IH(P) & WB.
EIA: 1:200.

IFA: 1:200-1:400.

Immunohistochemistry on formalin-fixed paraffin-embedded tissue sections: 1:50-1:100. High temperature citrate buffer antigen retrieval is highly recommended.

Western Blot: 1:1,000.

Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Physical form

Format: Purified
Liquid in 20mM Phosphate buffer, pH7.6, and 0.25M NaCl with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Parvovirus positive patient sample

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Roles of E4orf6 and VA I RNA in adenovirus-mediated stimulation of human parvovirus B19 DNA replication and structural gene expression.
Winter, K; von Kietzell, K; Heilbronn, R; Pozzuto, T; Fechner, H; Weger, S
Journal of virology null
Neutralization of human parvovirus B19 by plasma and intravenous immunoglobulins.
Jens Modrof,Andreas Berting,Bjorn Tille,Andreas Klotz,Christina Forstner,Sandra Rieger et al.
Transfusion null
Detection of parvovirus B19 capsid proteins in testicular tissues.
Monica E Polcz,Laura A Adamson,Reva S Datar,Larry J Fowler,Jacqueline A Hobbs
Urology null
Safder S Ganaie et al.
Journal of virology, 92(8) (2018-02-14)
Human parvovirus B19 (B19V) expresses a single precursor mRNA (pre-mRNA), which undergoes alternative splicing and alternative polyadenylation to generate 12 viral mRNA transcripts that encode two structural proteins (VP1 and VP2) and three nonstructural proteins (NS1, 7.5-kDa protein, and 11-kDa
Recombinant viral-like particles of parvovirus B19 as antigen carriers of anthrax protective antigen.
Yoji Ogasawara, Georgios Amexis, Hiroki Yamaguchi, Sachiko Kajigaya, Stephan H Leppla, Neal S Young
In Vivo null

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