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Key Documents

MAB1934

Sigma-Aldrich

Anti-Fibronectin Antibody

CHEMICON®, mouse monoclonal, 784A2A6

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Fibronectin Antibody, cell binding peptide, clone 784A2A6, purified antibody, clone 784A2A6, Chemicon®

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

784A2A6, monoclonal

species reactivity

human, chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

UniProt accession no.

P02751

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

chicken ... Fn1(396133)
human ... FN1(2335)

Specificity

Reacts with the 11kd cell adhesive fragment and weakly inhibits cell binding to fibronectin (fibronectin mediated cell adhesion).

Immunogen

Epitope: cell binding peptide
Human plasma fibronectin.

Application

Anti-Fibronectin Antibody, cell binding peptide, clone 784A2A6 detects level of Fibronectin & has been published & validated for use in ELISA & WB.
ELISA

Microscopy

Western blotting at 1-5 μg/mL

Cellular Adhesion Studies

Note: Dilution without addition of carrier protein may cause denautration or adherence of the antibody to the vial. Dilution into a 1% bovine serum albumin containing buffer is recommended.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 6 months in undiluted aliquots

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Souhaila Ghadhab et al.
Journal of biomedical materials research. Part A, 109(11), 2187-2198 (2021-05-02)
Intraosseous transcutaneous amputation prosthesis is a new approach in orthopedic implants that overcomes socket prosthesis problems. Its long-term performance requires a tight skin-implant seal to prevent infections. In this study, fibronectin (Fn), a widely used adhesion protein, was adsorbed or
S Kim et al.
The Journal of biological chemistry, 275(43), 33920-33928 (2000-08-17)
Recent studies indicate that angiogenesis depends, in part, on ligation of integrin alpha(5)beta(1) by fibronectin. Evidence is now provided that integrin alpha(5)beta(1) regulates the function of integrin alpha(v)beta(3) on endothelial cells during their migration in vitro or angiogenesis in vivo.
Helena P Felgueiras et al.
Langmuir : the ACS journal of surfaces and colloids, 30(31), 9477-9483 (2014-07-24)
Functionalization of surfaces with poly(sodium styrenesulfonate) (poly(NaSS)) has recently been found to enhance osteointegration of implantable materials. Radical polymerization of poly(NaSS) on titanium (Ti)-based substrates has been used to improve their long-term performance by preventing fibrosis and consequently implant loosening.

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