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AP113P

Sigma-Aldrich

Goat Anti-Human IgG Antibody, Fc, HRP conjugate

Chemicon®, from goat

Synonym(s):

Anti-Human IgG HRP, Goat Anti-Human IgG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is the most common antibody isotype found in the human serum. It exists in four isotypes IgG1, IgG2, IgG3, and IgG4. IgG structure is characterized with four polypeptide chains with two identical heavy (H) chains and two identical λ light (L) chains. These chains are inter-linked by disulfide bonds. IgG is produced by the B cells.

Immunogen

Human IgG

Application

Goat Anti-Human IgG Antibody, Fc, HRP conjugate has been used in enzyme linked immunosorbent assay (ELISA)(1:3,000).
It is also suitable for use in:
  • enzyme immunoassay (EIA) with OPD: 1:5,000-1:100,000
  • immunohistochemistry 1:500-1:5,000
  • western blot

Biochem/physiol Actions

Immunoglobulin G (IgG) is implicated in antibody-dependent cell-mediated cytotoxicity (ADCC). It aids in the activation of the classical pathway of the complement system. It neutralizes virus particles and toxins. Maternal IgG is transferred to the fetus through the placenta that is crucial for protecting newborns against infectious diseases. Measurement of IgG levels in severe acute respiratory syndrome coronavirus (SARS‐CoV)‐2 infected patients can be used as a potential strategy to assess the severity and prognosis of coronavirus disease 2019 (COVID‐19).

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sally Esmail et al.
Cell reports methods, 1(2), 100011-100011 (2021-07-09)
We have developed a rapid, accurate, and cost-effective serologic test for SARS-CoV-2 virus, which caused the COVID-19 pandemic, on the basis of antibody-dependent agglutination of antigen-coated latex particles. When validated using plasma samples that are positive or negative for SARS-CoV-2
Tara Bancroft et al.
Cellular immunology, 304-305, 35-43 (2016-05-24)
The recent increase in cases of whooping cough among teenagers in the US suggests that the acellular Bordetella pertussis vaccine (aP) that became standard in the mid 1990s might be relatively less effective than the whole-bacteria formulation (wP) previously used
Margherita Passariello et al.
Scientific reports, 11(1), 11046-11046 (2021-05-28)
Among the therapies against the pandemic SARS-CoV-2 virus, monoclonal Antibodies (mAbs) targeting the Spike glycoprotein represent good candidates to interfere in the Spike/ACE2 interaction, preventing virus cell entry. Since anti-spike mAbs, used individually, might be unable to block the virus
PCSK9 prosegment chimera as novel inhibitors of LDLR degradation.
Saavedra, YG; Zhang, J; Seidah, NG
Testing null
Dorothee Winterberg et al.
Journal of clinical medicine, 10(12) (2021-07-03)
B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most frequent malignancy in children and also occurs in adulthood. Despite high cure rates, BCP-ALL chemotherapy can be highly toxic. This type of toxicity can most likely be reduced by antibody-based immunotherapy

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