ABT11
Anti-Fibroblast activation protein alpha (FAP) Antibody
from rabbit
Synonym(s):
fibroblast activation protein, alpha, fibroblast activation protein, alpha subunit, integral membrane serine protease, seprase, 170 kDa melanoma membrane-bound gelatinase, FAP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FAP(2191)
General description
Fibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.
Specificity
The antibody recognizes the extracellular domain of Fibroblast activation protein alpha
Immunogen
Epitope: Extracellular domain
Histidine-tagged recombinant protein corresponding to human FAP.
Application
Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.
Immunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002).
Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).
Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).
Research Category
Cell Structure
Apoptosis & Cancer
Cell Structure
Apoptosis & Cancer
Research Sub Category
ECM Proteins
Tumor Markers
ECM Proteins
Tumor Markers
Quality
Evaluated by Western Blot in HEK-FAP xenograft tumor lysate.
Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.
Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.
Target description
~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa It is possible that both bands may be obserbved in certain cell lines.
Physical form
Ammonium Sulfate
Format: Purified
Purified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK-FAP xenograft tumor lysate
HEK-FAP xenograft tumor lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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