This product is comprised of stabilized gold nanoparticles in 0.1 mM PBS. There is no mannan present. The material may be concentrated by centrifugation. Note that aggregation may become an issue if the particles are removed from the stabilizing solution.
753610
Gold nanoparticles
20 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, reactant free
Synonym(s):
Au NP, Gold colloid
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About This Item
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Quality Level
form
nanoparticles
suspension
concentration
~6.54E+11 particles/mL
OD
1
diameter
20 nm
λmax
518-522 nm
PDI
<0.2
storage temp.
2-8°C
SMILES string
[Au]
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
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General description
Gold nanoparticles (AuNPs), 20 nm are colloidal nanomaterials that have quasi-dynamic nature. Their properties include high stability, high surface area, good conductivity, and low toxicity. Their surface chemistry and biocompatibility allow them to be useful in biological applications.[1][2][3][4][5][6]
Application
Legal Information
Product of CytoDiagnostics, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Steven J. Oldenburg, Ph.D. provides an overview of lateral flow diagnostic assays and discusses the use of ultra-bright reporter particles based on the unique optical properties of gold nanoshells that significantly increase the sensitivity of lateral flow immunoassays.
Steven J. Oldenburg, Ph.D. provides an overview of lateral flow diagnostic assays and discusses the use of ultra-bright reporter particles based on the unique optical properties of gold nanoshells that significantly increase the sensitivity of lateral flow immunoassays.
Sustainable energy sources with high production efficiency are crucial for meeting increasing energy demand.
Steven J. Oldenburg, Ph.D. provides an overview of lateral flow diagnostic assays and discusses the use of ultra-bright reporter particles based on the unique optical properties of gold nanoshells that significantly increase the sensitivity of lateral flow immunoassays.
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If i want to concentrate or isolate gold mannan particles and remove any unlabeled mannan what filtration or centrifugation conditions should be used.
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Can the Gold nanoparticles be frozen?
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No, the Gold nanoparticles should not be frozen. If frozen, the gold nanoparticles will irreversibly aggregate, turning the solution clear.
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Why does my gold nanoparticle solution turn violet when I add salt containing buffer?
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Due to repulsive forces arising from the surface charge of gold nanoparticles, an energy barrier must be overcome for individual particles to interact. When no (or small) amounts of electrolytes such as NaCl is present, this energy barrier is too strong for interaction to occur between particles. However, upon addition of NaCl this energy barrier is reduced allowing the gold nanoparticles to interact and aggregate. This aggregation causes a phenomenon called surface-plasmon coupling which changes the adsorption maximum of light to a higher wavelength resulting in a change in color of the solution.
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Do I need to wash my gold nanoparticles?
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For most applications, our gold nanoparticles can be used without any additional washing steps. If you have a sensitive application that requires additional washing the best way to do so is by either centrifugation or filtration.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the shape of the gold nanoparticles?
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The majority (>95%) of the gold nanoparticles are spherical.
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How do you attach gold nanoparticles to glass?
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To view the procedure on how to attach gold nanoparticles to glass please view information in the reference - Journal of Atomic, Molecular, and Optical PhysicsVolume 2012 (2012). Article ID 683830, 6 pagesdoi:10.1155/2012/683830
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Which gold nanoparticle size should I choose for my application?
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The size of gold nanoparticle to use is very depandent upon the intended application. Generally, smaller particles offer better sensitivity in applications such as immunogold labeling due to less steric hindrance and the ability to bind more gold nanoparticles to the desired target. Small gold nanoparticles are less visible than larger particles, however, which must also be taken into account.As an application example, particles with a size between 30-50nm are particularly useful for the development of rapid tests such as lateral flow assays.
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How do you attach gold nanoparticles to peptides and peptide conjugates?
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To view information on how to attach gold nanoparticles to peptides and peptide conjugates, please view the information in the reference - Rosenthal, S. J. and Wright, D. W. (Eds.). (2005), p. 91-92. NanoBiotechnology Protocols. New Jersey. Humana Press.
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