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G1007465

HPLC Analysis of Alfuzosin and Related Substances on Chromolith® HighResolution RP-18 endcapped

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

sample preparation: Dissolve 40.0 mg of the sample in 100 mL of mobile phase. 0.4 mg/mL (400 ppm) of USP Alfuzosin System Suitability Mixture RS in Mobile phase
column: Chromolith® HighResolution RP-18 endcapped, 150 x 4.6 mm (1.52023.0001)
mobile phase: [A] 5 ml of perchloric acid in 900 mL Milli-Q water. Adjust pH to 3.5 with 2M NaOH. Diluter to 1000 mL with water; [B] acetonitrile; [C] THF; (80:20:1, A:B:C)
flow rate: 1 mL/min
pressure: 994 psi (69 Bar)
column temp.: 25 °C
detector: 254 nm cell: 10 μL
injection: 10 μL
sample: 0.40 μg/mL of Alfuzosin Hydrochloride in Mobile phase from the Sample solution

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Deacylated Alfuzosin
    Alfuzosin
    Furamide analog

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