G001635
HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX
application for SPE, application for HPLC
About This Item
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: urine spiked with 3- and 4-methylpyrazole at 1 mg/mL
SPE tube/cartridge: Discovery DSC-SCX, 500 mg/3 mL (52686-U)
condition: 2 mL methanol; 2 mL DI water
sample addition: 1 mL
washing: 2 mL DI water
elution: 2 mL 5% methanol in 250 mM potassium phosphate buffer, pH 7.4
column: Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm guard column and 0.5 μm frit filter (504955)
mobile phase: MeOH:5 mM phosphate buffer, pH 6 (20:80)
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 220 nm
injection: 25 μL, dilute urine extract
suitability
application for HPLC
application for SPE
application(s)
forensics and toxicology
Legal Information
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analytical column
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