567516-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
Discovery Pentafluorophenyl PFP HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
5 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Application
- Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.: This study leverages the Discovery® HS F5 HPLC Column for advanced arsenic speciation in rice, enhancing food safety analysis and ensuring compliance with health regulations (Baba et al., 2014).
- High-performance liquid chromatographic determination of tiapride and its phase I metabolite in blood plasma using tandem UV photodiode-array and fluorescence detection.: Demonstrates the versatility of the Discovery® HS F5 HPLC Column in the sensitive and simultaneous detection of tiapride and its metabolites, contributing to therapeutic drug monitoring and pharmacokinetic studies (Nobilis et al., 2011).
- Simultaneous quantification of nicotine and metabolites in rat brain by liquid chromatography-tandem mass spectrometry.: Utilizes the Discovery® HS F5 HPLC Column to accurately measure nicotine and its metabolites in rat brain, providing crucial data for studies on addiction and neuropharmacology (Vieira-Brock et al., 2011).
- Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.: This research uses the Discovery® HS F5 HPLC Column to identify biomarkers in various biological samples from diabetic mice, enhancing our understanding of diabetes at the molecular level (Tsutsui et al., 2011).
- Practical analytical approach for the identification of biomarker candidates in prediabetic state based upon metabonomic study by ultraperformance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry.: Employs the Discovery® HS F5 HPLC Column in a metabolomic approach to identify early biomarkers of diabetes, advancing preventive medicine and metabolic research (Tsutsui et al., 2010).
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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