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Ascentis® Express 90 Å RP-Amide (2 μm) HPLC Columns

L × I.D. 10 cm × 3 mm UHPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Ascentis® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 3 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi)
60 °C temp. range

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 3 mm

surface area

120 m2/g

impurities

<5 ppm (metals)

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

amide, alkyl phase

particle size

2 μm

pore size

90 Å pore size

pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis® Express 2 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on a new Fused-Core® particle design. The fused core partical provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 micron thick porous shell and the small overall particle size of 2-microns. The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Certificates of Analysis (COA)

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USPIO001278
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USPIO001267

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Martin Mzik et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1020, 85-89 (2016-04-01)
A simple, rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the quantitative determination in rat plasma of a new candidate for AD treatment, namely PC 48 (a 7-MEOTA-donepezil like compound) in
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
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