T8819

TFIIB human

~5 units/μL, recombinant, expressed in E. coli, solution

• MDL number: MFCD01636087
• UNSPSC Code: 12352202
• NACRES: NA.56

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: solution
• mol wt: ~32 kDa
• concentration: ~5 units/μL
• UniProt accession no.: Q00403
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... GTF2B(2959)

Description

Biochem/physiol Actions

General transcription factor for RNA Polymerase II.

The amino terminal region of TFIIB contains a zinc ribbon fold composed of antiparallel Β strands. Mutational studies show that this domain is required for recruitment of RNA Pol II for transcriptional initiation.

Unit Definition

One gel shift unit is the amount of TFIIB required to form 0.2 pmol of TFIIB/TFIID/DNA complex as detected by gel shift.

Physical form

Solution in 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 0.1% Triton® X-100.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves