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T1200

Sigma-Aldrich

Anti-Tal (FQ-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Tsg101-associated ligase

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About This Item

MDL number:
MFCD06798551
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 84 kDa

species reactivity

human, mouse, rat

technique(s)

immunoprecipitation (IP): 20-40 μg using mouse brain S1 cytosolic fraction
indirect immunofluorescence: 10-20 μg/mL using mouse fibroblast NIH3T3 cell line
microarray: suitable
western blot: 2-4 μg/mL using whole extract of human kidney 293 cells expressing human Tal

UniProt accession no.

Q6UWE0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LRSAM1(90678)
mouse ... Lrsam1(227738)

General description

The gene LRSAM1 (leucine-rich repeat and sterile a motif-containing protein 1) is mapped to human chromosome 9q33.3. The protein is predicted to contain a leucine-rich repeat (LRR), an ezrin-radixin-moezin (ERM) domain, a coiled-coil (CC) region, a SAM (sterile α motif) domain and a carboxyl-terminal C3HC4-type RING (really interesting new gene) finger domain.

Immunogen

synthetic peptide encoding amino acids 5-21 located near the N-terminus of human Tal, conjugated to KLH. This sequence is identical in mouse and rat Tal.

Biochem/physiol Actions

LRSAM1 (leucine-rich repeat and sterile a motif-containing protein 1) is an E3 ligase. It binds and ubiquitinates Tsg101 (tumor susceptibility gene 101), inactivating Tsg101-mediated sorting of epidermal growth factor receptors and viral proteins. It also protects cytoplasm from invasive pathogens by participating in ubiquitination associated with intracellular bacteria. Mutations in LRSAM1 are linked with Charcot-Marie-Tooth disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
Amit I, et al.
Genes & Development, 18(14), 1737-1752 (2004)
Application of targeted multi-gene panel testing for the diagnosis of inherited peripheral neuropathy provides a high diagnostic yield with unexpected phenotype-genotype variability.
Antoniadi T, et al.
BMC Medical Genetics, 16(1), 84-84 (2015)
Laurent P Bogdanik et al.
Disease models & mechanisms, 6(3), 780-792 (2013-03-23)
Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous condition characterized by peripheral axon degeneration with subsequent motor and sensory deficits. Several CMT gene products function in endosomal sorting and trafficking to the lysosome, suggesting that defects in this cellular
Alan Huett et al.
Cell host & microbe, 12(6), 778-790 (2012-12-19)
Several species of pathogenic bacteria replicate within an intracellular vacuolar niche. Bacteria that escape into the cytosol are captured by the autophagic pathway and targeted for lysosomal degradation, representing a defense against bacterial exploitation of the host cytosol. Autophagic capture of

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