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SRP6549

Sigma-Aldrich

Alkaline Phosphatase from calf intestine

≥98% (SDS-PAGE)

Synonym(s):

ALP, ALP-1, ALPP, ALPPL, ALPPL2, PALP, PLAP, PLAP-1

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About This Item

EC Number:
232-631-4
UNSPSC Code:
12352205

biological source

bovine (calf) intestine

Assay

≥98% (SDS-PAGE)

form

freeze-dried protein glass

packaging

pkg of 1 mg
pkg of 10 μg
pkg of 50 μg

impurities

3000-6,000 EU/μg endotoxin (LAL test)

UniProt accession no.

P09487

shipped in

wet ice

storage temp.

−20°C

Gene Information

bovine (calf) ... ALPL(280994)

General description

Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa. The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.

Application

Alkaline phosphatase from calf intestine has been used as a standard in alkaline phosphatase assay.

Biochem/physiol Actions

Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids. It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.

Physical form

Lyophilized without any additives.

Reconstitution

Reconstitute in distilled water or dilute buffer.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Affinity-chromatography purification of alkaline phosphatase from calf intestine.
Brenna O
The Biochemical Journal, 151, 291-296 (1975)
Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity.
Addison WN
The Journal of Biological Chemistry, 282, 15872-15883 (2007)
Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion.
Pal S
Lab on a chip, 8, 1332-1341 (2008)
Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota.
Cell host & microbe, 2, 371-382 (2007)
Intestinal alkaline phosphatase. Physical properties and quaternary structure.
M Fosset et al.
Biochemistry, 13(9), 1783-1788 (1974-04-23)

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