SRP3194

CTACK (CCL27) from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): ALP, CCL27, Cuteaneous T-cell Attracting Chemokine, Skinkine
• UNSPSC Code: 12352200

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 10.0-100.0 ng/mL
• mol wt: 10.9 kDa
• packaging: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: off-white to yellow
• UniProt accession no.: Q9Z1X0
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... CCL27(20301)

Description

General description

CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant murine CTACK is a 10.9 kDa protein containing 95 amino acid residues.

Biochem/physiol Actions

CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Recombinant murine CTACK is a 10.9 kDa protein containing 95 amino acid residues.

Sequence

LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable