SRP0416

T4 Beta-glucosyltransferase

recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

• Synonym(s): T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: Escherichia coli
• recombinant: expressed in E. coli
• Assay: ≥83% (SDS-PAGE)
• form: aqueous solution
• mol wt: 41.6 kDa
• packaging: pkg of 100 μg
• NCBI accession no.: NP_049658
• UniProt accession no.: P04547
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: bacteriophage T4 ... B-GT(1258765)

Description

General description

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

Application

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Biochem/physiol Actions

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg²⁺, Mn²⁺ and Ca²⁺ activate this enzyme.

Physical form

Formulated in 200 mM imidazole and 20% glycerol.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H360D
• Precautionary Statements: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable