SRP0149

Set7/Set9 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(s): KMT7, Lysine N-methyltransferase 7, SET domain containing (lysine methyltransferase) 7, SET7/9, SETD7
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• mol wt: 61 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_030648
• UniProt accession no.: Q8WTS6
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SETD7(80854)

Description

General description

Human recombinant SET7/9, amino acids 52-end, with N-terminal GST tag, GenBank Accession No. NM_030648, expressed in a E. coli expression system. MW = 61 kDa,

SET7/9 also known as KMT7/SET domain containing lysine methyltransferase 7 (SETD7), is encoded by the gene mapped to human chromosome 4q31.1. The encoded protein belongs to SET domain-containing protein family.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

SET7/9 acts as a histone methyltransferase and catalyzes monomethylation of histone H3 at lysine 4 (H3K4me1) in vitro. Down-regulation of SET7/9 expression affects human embryonic stem cell differentiation. The encoded protein is implicated in the regulation of proteins and transcription factor activity. It also stimulates promoters of methylation-dependent co-recruitment through non-histone protein methylation. Mutation in the gene encoding SET7/9 is associated with serum lycopene concentrations. Elevated expression of the gene is associated with progression of hepatocellular carcinoma (HCC).

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable