SAB4200830

Anti-Staphylococcal Enterotoxin A-Peroxidase antibody produced in rabbit

IgG fraction of antiserum

• Synonym(s): Enterotoxin type A, SEA
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: peroxidase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• description: Research area: Microbiome
• form: lyophilized powder
• mol wt: 25-30 kDa
• species reactivity: Staphylococcus aureus
• concentration: ~4 mg/mL
• technique(s): direct ELISA: 1:2,000-1:4,000 (recommended using 1 μg/ml Staphylococcal Enterotoxin A for coating.)
• UniProt accession no.: P0A0L2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Staphylococcus aureus ... sea(22112712)

Description

General description

Staphylococcus aureus is a Gram positive bacterium, that cause diseases in human population including food poisoning and toxic shock syndrome. S. aureus expresses several secreted virulence factors, such as various enzymes, cytotoxins, exotoxins, and exfoliative toxins. Exotoxins include more than 20 serologically classified staphylococcal enterotoxins (SEs), the best characterize are the SEs A, B through V and toxic shock syndrome toxin-1 (TSST-1), these enterotoxins are similar in activity, sequence, structure and molecular weight (~25-30 kDa).
Mainly SEA but also SEB are known as superantigens due to their ability to bind class II MHC molecules on antigen presenting cells and stimulate extensive T-cells activation followed by massive cytokine release leading to an acute toxic shock.
SE proteins have a significant resistance to heat and acid, it is also resistant to gastrointestinal proteases including pepsin, trypsin, rennin and papain. Thus, killing the S. aureus bacteria may not be sufficient to eliminate these superantigens risk from causing food poisoning.
As described in various researches, detection of SEA contamination is a major challenge in the food industry, including milk and cooked food products.
SEA high affinity to MHC class II receptors on antigen presenting cells and tissues, followed by the excessive systemic immune activation, was applied in attempts to use bispecific antibodies (BsAbs) genetically fused to SEA as a T-cell stimulator. The SEA activation resulted in a non-specific activation of MHC class II-positive tissues which causes side-effects. Mutated SEA, D227A was developed to reduce the adverse effects for directing activated T-cells toward tumor cells.

Immunogen

Purified toxin from Staphylococcus aureus, (This toxin is coded by a bacteriophage).

Application

The antibody may be used in various immunochemical techniques including ELISA.

Physical form

Supplied as a lyophilized powder.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2