SAB4200527

Anti-USP8 antibody, Mouse monoclonal

clone US872, purified from hybridoma cell culture

• Synonym(s): Anti-Deubiquitinating enzyme 8, Anti-HumORF8, Anti-UBPY, Anti-Ubiquitin carboxyl-terminal hydrolase 8, Anti-Ubiquitin isopeptidase Y, Anti-Ubiquitin specific peptidase 8, Anti-Ubiquitin thioesterase 8, Anti-Ubiquitin-specific-processing protease 8, Anti-hUBPy, Monoclonal Anti-USP8 antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: US872, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~127 kDa; antigen ~70 kDa
• species reactivity: human, rat, bovine, monkey, mouse, dog
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: 5-10 μg/test using MCF7 cells; indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells; western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts
• UniProt accession no.: P40818
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... USP8(9101)

Description

General description

Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs).

Immunogen

synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH.

Application

Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry.

Biochem/physiol Actions

Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable