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SAB4200249

Sigma-Aldrich

Anti-PTPN2 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-PTN2, Anti-PTPT, Anti-TC-PTP, Anti-TCELLPTP, Anti-TCPTP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~48 kDa

species reactivity

mouse, rat, human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 1.5-3.0 μg/mL using Jurkat cell extracts, rat kidney extracts (S1 fraction) or mouse kidney extracts (S1 fraction).

UniProt accession no.

P17706

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PTPN2(5771)

General description

Protein tyrosine phosphatase non-receptor type 2 (PTPN2), also known as T cell protein tyrosine phosphatase (TCPTP), is an intracellular nonreceptor tyrosine-specific phosphatase that is expressed ubiquitously at various stages of mammalian development. PTPN2 exists as two alternatively spliced variants, PTPN2 isoform 1 (p48TC, 48 kDa) that is targeted to the endoplasmic reticulum by a hydrophobic C-terminal region and a shorter PTPN2 isoform 2 (p45TC, 45 kDa) that is targeted to the nucleus.

Immunogen

synthetic peptide corresponding to a sequence located near the C-terminus of human PTPN2, conjugated to KLH. The corresponding sequence is identical in human PTPN2 isoform 2 and highly conserved (single amino acid substitution) in mouse PTPN2.

Application

Anti-PTPN2 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunostaining

Biochem/physiol Actions

PTPN2 acts on several cytoplasmic substrates including epidermal growth factor receptor (EGFR), insulin receptor (InsR), Shc, janus kinases (JAKs), and the nuclear substrate signal transducer and activator of transcription 1 (STAT1). PTPN2 has been associated with type 1 diabetes.
Protein tyrosine phosphatase non-receptor type 2 (PTPN2) is a tyrosine phosphatase that negatively regulates tyrosine kinase and JAK/STAT signaling pathways. PTPN2 inhibits oncogenic JAK1 and thereby functions as a tumor suppressor in T-cell malignancies. Genetic alterations in PTPN2 have been associated with the risk of Crohn′s disease and ulcerative colitis .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication
Li H, et al.
Journal of Cell Science, 127(15), 3269-3279 (2014)
Jürgen Glas et al.
PloS one, 7(3), e33682-e33682 (2012-03-30)
Genome-wide association studies identified PTPN2 (protein tyrosine phosphatase, non-receptor type 2) as susceptibility gene for inflammatory bowel diseases (IBD). However, the exact role of PTPN2 in Crohn's disease (CD) and ulcerative colitis (UC) and its phenotypic effect are unclear. We
Maria Kleppe et al.
Nature genetics, 42(6), 530-535 (2010-05-18)
PTPN2 (protein tyrosine phosphatase non-receptor type 2, also known as TC-PTP) is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. In agreement with its role in the regulation
Maria Kleppe et al.
Haematologica, 96(11), 1723-1727 (2011-07-28)
We recently reported deletion of the protein tyrosine phosphatase gene PTPN2 in T-cell acute lymphoblastic leukemia. Functional analyses confirmed that PTPN2 acts as classical tumor suppressor repressing the proliferation of T cells, in part through inhibition of JAK/STAT signaling. We
T-cell protein tyrosine phosphatase (TCPTP) is irreversibly inhibited by etoposide-quinone, a reactive metabolite of the chemotherapy drug etoposide
Qing N, et al.
Molecular Pharmacology (2019)
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