All Photos(2)

Documents

SAB4200031

Anti-TNPO2 (C-terminal) antibody produced in rabbit

Name: Anti-TNPO2 (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Importin 3, Anti-Karyopherin beta-2b, Anti-Transportin-2

Sign Into View Organizational & Contract Pricing

All Photos(2)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing human TNPO2
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human TNPO2
western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human TNPO2

UniProt accession no.

O14787

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNPO2(30000)

General description

Transportin 2 (TNPO2 or TRN2) is a member of the karyopherin family of transport receptors. This protein exists in two isoforms TRN2a and TRN2b. The TNPO2 gene is located on human chromosome 19p13.2.

Specificity

Anti-TNPO2 (C-terminal) recognizes human TNPO2 isoform b.

Application

Anti-TNPO2 (C-terminal) antibody produced in rabbit may be used in:

immunoblotting
immunoprecipitation
immunofluorescence

Biochem/physiol Actions

Transportin 2 (TNPO2) is involved in a novel nuclear protein import and export pathway. This protein specifically binds to M9-containing proteins such as heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) as well as the nucleocytoplasmic shuttling sequence (HNS) region of HuR, but not to the classical nuclear localization signal (NLS)-containing proteins. TNPO2 protein interacts with a similar set of RNA-binding proteins in an RAs-related nuclear protein (Ran)- guanosine triphosphate (GTP) sensitive manner.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Transportin2 functions as importin and mediates nuclear import of HuR.

Stephan Güttinger et al.

Proceedings of the National Academy of Sciences of the United States of America, 101(9), 2918-2923 (2004-02-26)

The RanGTP-binding nuclear transport receptors transportin1 (TRN1) and transportin2 (TRN2) are highly similar in sequence but are reported to function in nuclear import and export, respectively. Here we show that TRN2 possesses properties of a nuclear import receptor. TRN1/2 both

Transportins 1 and 2 are redundant nuclear import factors for hnRNP A1 and HuR.

Ana Rebane et al.

RNA (New York, N.Y.), 10(4), 590-599 (2004-03-24)

Several mRNA-binding proteins, including hnRNP A1 and HuR, contain bidirectional transport signals that mediate both their nuclear import and export. Previously, Transportin 1 (Trn1) was identified as a mediator of hnRNP A1 import, whereas the closely related protein Transportin 2

Gene expression analysis reveals schizophrenia-associated dysregulation of immune pathways in peripheral blood mononuclear cells.

Erin J Gardiner et al.

Journal of psychiatric research, 47(4), 425-437 (2012-12-12)

Peripheral blood mononuclear cells (PBMCs) represent an accessible tissue source for gene expression profiling in schizophrenia that could provide insight into the molecular basis of the disorder. This study used the Illumina HT_12 microarray platform and quantitative real time PCR

IPO3-mediated Nonclassical Nuclear Import of NF-κB Essential Modulator (NEMO) Drives DNA Damage-dependent NF-κB Activation.

Byounghoon Hwang et al.

The Journal of biological chemistry, 290(29), 17967-17984 (2015-06-11)

Activation of IκB kinase (IKK) and NF-κB by genotoxic stresses modulates apoptotic responses and production of inflammatory mediators, thereby contributing to therapy resistance and premature aging. We previously reported that genotoxic agents induce nuclear localization of NF-κB essential modulator (NEMO)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service