High purity cellulose powders for partition chromatography.
Makes extremely durable layers. Sharp separations for amino acids, sugars and other hydrophilic substances. Development time as short as 2 hours for 8′′ plates.
Sigmacell cellulose is used in thin layer chromatography (TLC) and cellulose TLC adsorbents. Sigmacell cellulose has been used in studies to inform industrial applications for large-scale purification of enzymes as well as to investigate the cellulase system of Penicillium echinulatum, which has been identified as a potential cellulase producer for bioconversion processes.
Reconstitution
For TLC, simply blend a 15-20% aqueous slurry for about one minute and coat plates; dry at room temperature. Usually does not require activation before use.
The Journal of experimental biology, 207(Pt 23), 4095-4104 (2004-10-23)
Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes; endo-beta-1,4-glucanase and beta-1,4-glucosidase. These enzymes hydrolysed native cellulose to glucose. The digestive juice of both species also
Trends in plant science, 17(11), 666-674 (2012-07-13)
Directed plant cell growth is governed by deposition and alterations of cell wall components under turgor pressure. A key regulatory element of anisotropic growth, and hence cell shape, is the directional deposition of cellulose microfibrils. The microfibrils are synthesized by
Methylation of mercury (Hg) to highly toxic methyl Hg (MeHg), a process known to occur when organic matter (OM) decomposition leads to anoxia, is considered a worldwide threat to aquatic ecosystems and human health. We measured temporal and spatial variations
Most of the lineages of bacteria have remained unknown beyond environmental surveys using molecular markers. Until the recent characterisation of several strains, the phylum Armatimonadetes (formerly known as 'candidate division OP10') was a dominant and globally-distributed lineage within this 'uncultured
Proceedings of the National Academy of Sciences of the United States of America, 110(44), 17856-17861 (2013-10-16)
Cellulose is a linear extracellular polysaccharide. It is synthesized by membrane-embedded glycosyltransferases that processively polymerize UDP-activated glucose. Polymer synthesis is coupled to membrane translocation through a channel formed by the cellulose synthase. Although eukaryotic cellulose synthases function in macromolecular complexes
To standardize an enzymatic assay procedure of cellulase.
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