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P4780

Sigma-Aldrich

TWEEN® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonym(s):

POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12161700
NACRES:
NA.25
Pricing and availability is not currently available.

description

non-ionic

Quality Level

form

viscous liquid

mol wt

average mol wt ~1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

CMC

0.012 mM

density

1.06 g/mL at 25 °C (lit.)
1.07 g/cm3 at 25 °C (lit.)

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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General description

TWEEN 80 is a polyethylene sorbitol ester[1] and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.[2]

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used:
  • in Listeria monocytogenes enumeration using filtration method[3]
  • as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion[4]
  • as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing[5]

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

>235.4 °F - closed cup

Flash Point(C)

> 113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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