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P4039

Sigma-Aldrich

Phospholipase C Peptide

greener alternative

lyophilized powder, Type XIV, ≥150 units/mg protein

Synonym(s):

Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase

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About This Item

CAS Number:
9001-86-9
Enzyme Commission number:
3.1.4.3 (BRENDA, IUBMB)
EC Number:
232-638-2
MDL number:
MFCD00131895
UNSPSC Code:
41106300
NACRES:
NA.32

product name

Phospholipase C from Clostridium perfringens (C. welchii), Type XIV, lyophilized powder, ≥150 units/mg protein

biological source

bacterial (Clostridium perfringens)

Quality Level

200

type

Type XIV

form

lyophilized powder

specific activity

≥150 units/mg protein

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Enabling,

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Application

Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.

Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder in buffered salts

Preparation Note

Chromatographically purified

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Angela Buys et al.
Acta veterinaria Scandinavica, 62(1), 57-57 (2020-10-09)
An overall increase in poaching of white rhinoceros results in captive breeding becoming a significant component of white rhinoceros conservation. However, this type of conservation comes with its own difficulties. When wildlife is captured, transported and/or confined to a boma
In vitro inhibitory effect of hen egg white lysozyme on Clostridium perfringens type A associated with broiler necrotic enteritis and its alpha-toxin production
Zhang G, et al.
Letters in Applied Microbiology, 42(2), 138-143 (2006)
Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry
McCourt MT, et al.
Veterinary Microbiology, 106(3-4), 259-264 (2005)
G Zhang et al.
Letters in applied microbiology, 42(2), 138-143 (2006-01-31)
Clostridium perfringens type A causes both clinical and subclinical forms of necrotic enteritis in domestic avian species. In this study the inhibitory effect of hen egg white lysozyme on the vegetative form of Cl. perfringens type A and the production
Hrvoje Banfic et al.
The Journal of biological chemistry, 288(3), 1717-1725 (2012-11-28)
Several studies have demonstrated the activation of phosphoinositide-specific phospholipase C (Plc) in nuclei of mammalian cells during synchronous progression through the cell cycle, but the downstream targets of Plc-generated inositol 1,4,5-trisphosphate are poorly described. Phospholipid signaling in the budding yeast
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