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P4020

Sigma-Aldrich

Protamine sulfate

amorphous powder

Synonym(s):

Salmine, Salmine from salmon

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About This Item

CAS Number:
53597-25-4
MDL number:
MFCD00132091
UNSPSC Code:
12352202
NACRES:
NA.77
Pricing and availability is not currently available.

Product Name

Protamine sulfate salt from salmon, Grade X, amorphous powder

type

Grade X

Quality Level

200

form

amorphous powder

impurities

histone, free (Millon test)

color

white to off-white

solubility

6 M HCl: 25 mg/mL

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General description

Protamine are proteins rich in cysteine and arginine.[1] It possesses many phosphorylation sites.[2] It is found to be localized to the sperm head.[3]

Application

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine sulfate salt from salmon has been used:
  • in the pretreament of cells to study its effect on respiratory syncytial virus infection[4]
  • in lentivirus production[5]
  • to derivatize it into a diazeniumdiolate NO donor[6]

Biochem/physiol Actions

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000371992
0000371991
0000371992
0000371991
0000321774

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Sigma-Aldrich

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The design and evaluation of heparin-binding foldamers.
Sungwook Choi et al.
Angewandte Chemie (International ed. in English), 44(41), 6685-6689 (2005-08-12)
Microarrays of lentiviruses for gene function screens in immortalized and primary cells
Bailey SN, et al.
Nature Methods, 3(2), 117-117 (2006)
Wenjie Ouyang et al.
Human gene therapy, 32(9-10), 481-494 (2020-12-02)
β-Thalassemia is one of the most prevalent genetic diseases worldwide. The current treatment for β-thalassemia is allogeneic hematopoietic stem cell transplantation, which is limited due to lack of matched donors. Gene therapy has been developed as an alternative therapeutic option
Diazeniumdiolation of protamine sulfate reverses mitogenic effects on smooth muscle cells and fibroblasts
Van Lith R, et al.
Free radical biology & medicine, 82, 13-21 (2015)
Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection
Hallak LK, et al.
Virology, 271(2), 264-275 (2000)
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Questions

1–2 of 2 Questions  

  1. Are there other solvents besides 6M HCl to bring protamine sulfate salt into solution?

    1 answer

    1. This product is also soluble in water at 10mg/ml. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/195/258/p4020pis.pdf

      Helpful?

  2. Bonjour, Pourriez vous me confirmer qu'il s'agit bien d'un polypeptide et de combien d'unité de monomère est il formé ? Pourriez vous m'envoyer la représentation de la molécule ? merci

    1 answer

    1. Unfortunately, structural studies on for this product have not been performed. However, regarding structure characterization and molecular representation of protamine from salmon, the following publication may be of interest for reference.

      J Biol Chem. 1959 Sep:234:2314-6.
      Physical and chemical properties of protamine from the sperm of salmon (Oncorhynchus tschawytscha). II. Anion binding characteristics
      https://pubmed.ncbi.nlm.nih.gov/13807973/

      Helpful?

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