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P350-05

Sigma-Aldrich

Porcine Coronary Artery Smooth Muscle Cells: PCASMC (Cryovial)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

Porcine coronary artery

Quality Level

100

form

solid

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 38

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

diabetes; stroke; cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

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PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

vascular research, supply of blood to heart muscle

Components

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PCASMC Culture Protocol.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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In Young Choi et al.
Biomaterials, 269, 120222-120222 (2020-08-02)
Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation

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