N4787

Nek2, Active human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): Never In Mitosis Gene A-Related Kinase 2, Nima-Related Kinase 2
• MDL number: MFCD07370971
• UNSPSC Code: 51111800
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥85% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• mol wt: protein apparent mol wt ~80 kDa
• UniProt accession no.: P51955
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... NEK2(4751)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem/physiol Actions

Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.

Unit Definition

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable