The protocol is solely validated for use with plasma samples. Regrettably, there is no available data regarding its use with culture cell medium or other sample types. Experimental validation would be necessary. Additionally, considering that the kit has a linear range of 0.5 to 20 μM, it may be essential to optimize the required sample amount. However, it's important to note that guarantee can't be given for its performance.
MAK433
Adenosine Quantification Assay Kit
sufficient for 100 fluorometric tests
Synonym(s):
Adenosine Test Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
Quality Level
usage
sufficient for 100 fluorometric tests
input
plasma
detection method
fluorometric
relevant disease(s)
cancer; neurological disorders; cardiovascular diseases
shipped in
dry ice
storage temp.
−20°C
General description
Adenosine is a purine nucleoside base, which is the precursor of adenosine triphosphate, or ATP, and is utilized thoroughly throughout the entire body in general metabolism.[1] Extracellular adenosine acts as a local modulator with a generally cytoprotective function in the body.[2] Its effects on tissue protection and repair fall into four categories: increasing the ratio of oxygen supply to demand; protecting against ischaemic damage by cell conditioning; triggering anti-inflammatory responses; and the promotion of angiogenesis.[3]
Application
The Adenosine Quantification Assay Kit may be used for:
- Cancer Research
- Cardiovascular Disease Research
- Neurological Disease Research
Features and Benefits
- Supportive calculator (Click here to download a calculator excel file) Analyze results based on your experimental data!
- Quick instruction bench card - to assure your experimental success
- More experiments in one kit - contains sufficient reagents for 100 tests
Suitability
The kit is suitable for the quantification of adenosine in plasma.
Principle
The Adenosine Quantification Assay Kit provides a simple and quick fluorometric method for the detection of adenosine. The kit is based on a multi-step enzymatic approach, resulting in the formation of fluorescent product that is measured at λEx = 550 nm/λEm = 585. The kit has a linear range of 0 to 20 μM.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class Code
10 - Combustible liquids
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Find documentation for the products that you have recently purchased in the Document Library.
Adenosine receptors as therapeutic targets.
Jacobson K A, et al.
Nature Reviews. Drug Discovery, 5(3), 247-264 (2006)
B B Fredholm et al.
Pharmacological reviews, 53(4), 527-552 (2001-12-06)
Four adenosine receptors have been cloned and characterized from several mammalian species. The receptors are named adenosine A(1), A(2A), A(2B), and A(3). The A(2A) and A(2B) receptors preferably interact with members of the G(s) family of G proteins and the
Monika Samsel et al.
Postepy higieny i medycyny doswiadczalnej (Online), 67, 1189-1203 (2014-01-01)
Adenosine is an endogenous purine nucleoside that plays an important role in many biochemical processes. It acts through the four types of adenosine receptors: A1, A2A, A2B, A3 which are located i.a. in the immune, nervous, circulatory, respiratory or urinary
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Is it possible to use MAK433 with media samples instead of plasma? The product is specified for human plasma, but is it suitable for conditioned cell culture media?
1 answer-
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For the Adenosine Quantification Assay Kit (Cat# MAK433), does this also detect ATP/ADP or just adenosine?
1 answer-
MAK433-1KT Adenosine Quantification Assay Kit is specific for Adenosine. It does not measure ATP or ADP.
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Is that possible to measure adenosine on cell culture's supernatant?
1 answer-
This kit is intended for use with plasma. The end-user would have to determine suitability for use with cell culture supernatants. The lowest concentration used to generate the standard curve is 0.5 uM per well. Samples would have to have sufficient adenosine present for detection. See the link below to review the protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/126/mak433bul.pdfHelpful?
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혹시 이 제품이 abcam사에서 판매하는 Adenosine Assay Kit (Fluorometric) (ab211094) 제품과 동일하거나 비슷한 원리의 제품인지 알 수 있을까요?
1 answer-
Both the "Adenosine Quantification Assay Kit" (Product number: MAK433) and the "Adenosine Assay Kit (Fluorometric)" (ab211094) from Abcam are assay kits designed to measure adenosine levels using a multi-enzymatic method.
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