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MAK008

Sigma-Aldrich

Hydroxyproline Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Hydroxyproline Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

dermatological diseases

shipped in

wet ice

storage temp.

2-8°C

General description

Hydroxyproline (4-hydroxyproline) is a non-proteinogenic amino acid formed by the post-translational hydroxylation of proline. Hydroxyproline is a major component of collagen, where it serves to stabilize the helical structure. Because hydroxyproline is largely restricted to collagen, the measurement of hydroxylproline levels can be used as an indicator of collagen content. Conditions that increase collagen turnover can elevate serum and urine hydroxyproline levels. Urine and serum hydroxyproline levels can be used as a marker for bone resorption.

Application

Hydroxyproline Assay Kit has been used to determine the concentration of hydroxyproline and collagen.

Suitability

Suitable for hydroxyproline detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In the Hydroxyproline Assay Kit, hydroxyproline concentration is determined by the reaction of oxidized hydroxyproline with 4-(Dimethylamino)benzaldehyde (DMAB), which results in a colorimetric (560 nm) product, proportional to the hydroxyproline present.

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system, Thyroid

Supplementary Hazards

EUH031

Storage Class Code

5.1B - Oxidizing hazardous materials

Flash Point(F)

64.4 °F

Flash Point(C)

18 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Urinary biochemical markers for bone resorption during the menstrual cycle.
Gorai I, et al.
Calcified Tissue International, 57(2), 100-104 (1995)
Lodish H, et al.
Molecular Cell Biology. 4th edition. (2000)
Chunming Jiang et al.
BioMed research international, 2015, 867140-867140 (2016-02-18)
Acute kidney injury (AKI) is associated with an increased risk of developing advanced chronic kidney disease (CKD). Yet, effective interventions to prevent this conversion are unavailable for clinical practice. In this study, we examined the beneficial effects of Tanshinone IIA
Proline and hydroxyproline metabolism: implications for animal and human nutrition.
Wu G, et al.
Amino Acids, 40(4), 1053-1063 (2011)
M K North et al.
Meat science, 110, 145-152 (2015-08-01)
This study aimed to determine the optimum ageing period for vacuum-packed springbok (Antidorcas marsupialis) Longissimus thoracis et lumborum (LTL) and Biceps femoris (BF) muscle stored at 5.4 ± 1.0°C. Portions of muscle from seven male and six female springbok were
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