M8194

Anti-MAFF (C-Terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-v-maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Family, ProteinF
• MDL number: MFCD08705477
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 18 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 2-4 μL using A431 cell line; microarray: suitable; western blot: 1:2,000-1:4,000 using whole extracts of the A431 cell line
• UniProt accession no.: Q9ULX9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAFF(23764)

Description

General description

The MAFF gene is located on the human chromosome at 22q13.1. The encoded protein is a small MAF protein and is expressed in the nucleus.

v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) is a transcription factor which does not have a transactivation domain but possesses a leucine-zipper.

Specificity

Anti-MAFF (C-terminal) specifically recognizes MAFF.

Immunogen

synthetic peptide corresponding to amino acids 147-163 of human MAFF (isoform a). The immunizing peptide does not cross-react with other members of the family.

Application

Anti-MAFF (c-terminal) antibody produced in rabbit has been used in:

• western blotting[1]
• chromatin immunoprecipitation (chip)[1]
• co-immunoprecipitation[1]
• immunoprecipitation

Biochem/physiol Actions

The small MAF proteins are associated with many physiological processes including development, differentiation, hematopoiesis and stress response. Interestingly, these proteins regulate the stress response via different mechanisms. The MAFF gene is down-regulated in diabetes mellitus 1 patient. This gene is underexpressed in acute lymphoblastic lymphoma (ALL) patients.

v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) has been shown to interact with other transcription factors and it associates with certain regulatory sequences in the gene encoding the oxytocin receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves