L0881
Lectin from Arachis hypogaea (peanut)
lyophilized powder, Affinity-purified
Synonym(s):
PNA, Peanut agglutinin
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All Photos(2)
About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
form
lyophilized powder
Quality Level
potency
<0.1 μg per mL agglutination activity
quality
Affinity-purified
impurities
salt, free
storage temp.
−20°C
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General description
Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa.
Application
Lectin from Arachis hypogaea (peanut) has been used:
- to detect Thomson-Friedenreich antigen
- as a component in PBS to study its effects on cell viability
- to study its ability to block the binding site
Biochem/physiol Actions
Lectin from Arachis hypogaea (peanut) possess specificity towards β -D-Gal(1-3)-D-galNAc residue of Thomson − Friedenreich antigen. It is the most widely used diagnostic and prognostic marker for many squamous cell carcinomas.
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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