GE17-0756-05

Glutathione Sepharose^™ 4B

Cytiva 17-0756-05, pack of 100 mL

• Synonym(s): affinity chromatography resin, GST-tagged proteins purification
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• ligand: glutathione
• packaging: pack of 100 mL
• manufacturer/tradename: Cytiva 17-0756-05
• matrix: 4% agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning in place: 4-13
• working range: 4-13
• capacity: >25 mg binding capacity (horse liver GST/ml medium)

Description

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Glutathione Sepharose^™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose^™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Features and Benefits

• High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
• Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids