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GAHK20

Supelco

Glucose (HK) Assay Kit

sufficient for 20 assays

Synonym(s):

Glucose Quantification Kit

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About This Item

UNSPSC Code:
12164500
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 20 assays

Quality Level

100

analyte chemical class(es)

sugars (glucose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Related Categories

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.

Kit Components Only

Product No.
Description
  • glucose (HK) assay reagent 20 mL
  • glucose standard .5 mL

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000411005
0000411005
0000331189
0000351488
0000294939

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Daniela T Fuller et al.
Atherosclerosis, 293, 1-10 (2019-12-11)
Oxidative stress is associated with cardiometabolic traits in observational studies, yet the underlying causal relationship remains unclear. Apolipoprotein E-deficient (Apoe-/-) mice develop significant hyperlipidemia and hyperglycemia on a Western diet. Here we conducted linkage analysis to investigate genetic connections between
Bo-Ruei Chen et al.
Experimental gerontology, 44(8), 493-502 (2009-05-05)
We describe a new chronological lifespan (CLS) assay for the yeast Schizosaccharomyces pombe. Yeast CLS assays monitor the loss of cell viability in a culture over time, and this new assay shows a continuous decline in viability without detectable regrowth
Rebecca A Haeusler et al.
Nature communications, 5, 5190-5190 (2014-10-14)
Insulin integrates hepatic glucose and lipid metabolism, directing nutrients to storage as glycogen and triglyceride. In type 2 diabetes, levels of the former are low and the latter are exaggerated, posing a pathophysiologic and therapeutic conundrum. A branching model of
Kuei-Ching Hsiung et al.
Biomedical journal, 43(1), 32-43 (2020-03-24)
CDGSH iron sulfur domain-containing protein 1 (CISD-1) belongs to the CISD protein family that is evolutionary conserved across different species. In mammals, CISD-1 protein has been implicated in diseases such as cancers and diabetes. As a tractable model organism to
Helen H Tai et al.
Plant physiology and biochemistry : PPB, 146, 163-176 (2019-11-23)
Tubers are vegetative reproduction organs formed from underground extensions of the plant stem. Potato tubers are harvested and stored for months. Storage under cold temperatures of 2-4 °C is advantageous for supressing sprouting and diseases. However, development of reducing sugars can
View All Related Papers

Questions

1–10 of 13 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. The procedure says that solutions that are strongly colored should be decolorized for the kit to work. Do you have any suggestions on how to decolorize my sample?

    1 answer

    1. Solutions may be decolorized by adding a small amount of charcoal, stirring a few moments, and then clarifying by filtration or centrifugation to remove the charcoal. An activated charcoal product, such as item 161551 would be appropriate. See the link below to review this product:
      https://www.sigmaaldrich.com/product/sigald/161551

      Helpful?

  4. I want to detect the change of glucose concentration in macrophages. How to prepare the sample

    1 answer

    1. A macrophage sample should be lysed using a cell lysis protocol similar to one used for Western Blotting. A useful lysis protocol can be found linked below.

      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/western-blotting/sample-preparation

      Following cell lysis, the sample should be diluted with deionized water to 0.05 to 5 mg of glucose per mL. The sample should be deproteinized (using a common perchloric acid precipitation procedure) and filtered to clarify the solution. Any solutions that are strongly colored should be decolorized using a decolorization agent such as activated charcoal. Any samples in which gases are liberated during the sample preparation process should be degassed prior to analysis.

      Helpful?

  5. How to adapt the Glucose Assay Kit to a 96-well plate?

    1 answer

    1. It may be possible to adapt this kit for use in a 96-well plate format. This would require method development and reagent scale down by the end user. There are no specific instructions available. Should a 96-well assay be preferred or required, please see product MAK476 at the link below. This product has been optimized for use in this format.
      https://www.sigmaaldrich.com/product/sigma/mak476

      Helpful?

  6. Is the product G3293-20ML included in the GAHK20-1KT kit?

    1 answer

    1. Yes, the item G3293-20ML is a component of the complete GAHK20-1KT kit. Therefore, if G3293-20ML is on backorder, it can be obtained by ordering the GAHK20-1KT.

      Helpful?

  7. Is GAHK20 assay compatible with C. elegans worm lysates? What buffer you recommed to prepare lysates?

    1 answer

    1. The Glucose (HK) Assay Kit has been optimized for use in food and other crude materials. This kit has not been qualified for use with cells or organisms such as C. elegans. The researcher would have to determine suitability, however there is some indication that other customers have been successful. See the link below for a publication that may be of interest:
      https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1005561&type=printable

      Note that samples may require centrifugation, filtration or deproteinization to further clarify.

      Helpful?

  8. Can the procedure for Glucose (HK) Assay Kit, Product GAHK20, be scaled down?

    1 answer

    1. Although we have not done it, you should be able to scale down the procedure as long as you have pipettes capable of dispensing accurate ul volume quantities and an instrument that can monitor the 340 nm wavelength.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How do I decolorize a solution for use with Product GAHK20, Glucose (HK) Assay Kit?

    1 answer

    1. You can decolorize a solution by adding a small amount of charcoal to the solution, swirl, then filter or centrifuge to remove charcoal.

      Helpful?

1–10 of 13 Questions  

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