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G8404

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

Synonym(s):

Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH

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About This Item

CAS Number:
9001-40-5
Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

300

recombinant

expressed in E. coli

form

ammonium sulfate suspension

specific activity

≥550 units/mg protein (biuret)

mol wt

128 kDa

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

UPI0021A4D550

foreign activity

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

storage temp.

2-8°C

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General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.

Research area: Cell Signaling

Application

Glucose-6-phosphate dehydrogenase has been used:

  • to test ketose reductase activity in developing maize endosperm.
  • to determine the levels of mannose in coronary heart disease patient-derived serum
  • to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
  • to determine the glucose uptake in cultured human muscle satellite cells

Biochem/physiol Actions

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 containing 50 mM Tris and 1 mM MgCl2, pH 7.5

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Charles-David Dubé et al.
AMB Express, 9(1), 23-23 (2019-02-08)
Extracellular polymeric substances (EPS) play major roles in the efficacy of biofilms such as anaerobic granules, ranging from structural stability to more specific functions. The EPS of three granular anaerobic sludges of different origins were studied and compared. Particularly, the
Angelo Minucci et al.
IUBMB life, 61(1), 27-34 (2008-10-23)
Glucose 6-phosphate dehydrogenase (G6PD) deficiency is the most common defect of red blood cells. Although some different laboratory techniques or methods are employed for the biochemical screening, a strict relationship between biochemists, clinicians, and molecular biologists is necessary for a
Hemang M Parikh et al.
BMC endocrine disorders, 21(1), 32-32 (2021-03-01)
Insulin resistance (IR) in skeletal muscle is a key feature of the pre-diabetic state, hypertension, dyslipidemia, cardiovascular diseases and also predicts type 2 diabetes. However, the underlying molecular mechanisms are still poorly understood. To explore these mechanisms, we related global
Cara A Griffiths et al.
Nature, 540(7634), 574-578 (2016-12-16)
The pressing global issue of food insecurity due to population growth, diminishing land and variable climate can only be addressed in agriculture by improving both maximum crop yield potential and resilience. Genetic modification is one potential solution, but has yet
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

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