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G8021

Sigma-Aldrich

Monoclonal Anti-β-Galactosidase antibody produced in mouse

clone GAL-13, ascites fluid

Synonym(s):

Monoclonal Anti-β-Galactosidase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GAL-13, monoclonal

contains

15 mM sodium azide

species reactivity

bacteria

technique(s)

dot blot: suitable using soluble β-galactosidase
immunocytochemistry: suitable
indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Escherichia coli ... lacZ(1036228) , lacZ(1036228)
Escherichia coli K12 ... Lacz(945006)

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General description

Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.

Specificity

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

Immunogen

β-galactosidase from E. coli

Application

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:

  • immunostaining and histochemistry
  • immunostaining and microscopy
  • immunohistochemistry
  • immunofluorescence staining
  • enzyme linked immunosorbent assay(ELISA)
  • immuno dot blot

Biochem/physiol Actions

β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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