F0504

Folate binding protein from bovine milk

lyophilized powder

• Synonym(s): Folate Protein
• MDL number: MFCD00131098
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine milk
• Quality Level: 200
• form: lyophilized powder
• usage: 1 mg sufficient for ~4,000 assays
• composition: Protein, 20-40% modified Warburg-Christian
• technique(s): ligand binding assay: suitable; surface plasmon resonance (SPR): suitable
• capacity: 1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.)
• storage temp.: −20°C

Description

Application

Folate binding protein can be used in radioligand binding assays of serum folate. The folate receptor has been used in studies as a therapeutic target in a variety of cancers.

Folate binding protein from bovine milk has been used in folate binding studies using surface plasmon resonance and in the uptake studies in kidney proximal tubule cells.

Useful for radioligand binding assays of serum folate. May also bind N-methyltetrahydrofolic acid and other folate analogues.

Biochem/physiol Actions

Folate-binding protein (FBP) is present as a cell receptor and binds to folate in equimolar ratio. It is involved in the transport of folate and improves their bioavailability.

Packaging

Package size based on protein content

Quality

Purified over 1,000 fold. May contain a trace of β-mercaptoethanol.

Physical form

Lyophilized powder containing buffer salt as sodium citrate

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves