CCD 18Lu

90112706, human lung, Fibroblast

• UNSPSC Code: 41106514

Properties

• product name: CCD 18Lu, 90112706
• biological source: human lung
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human lung

Cell Line Description

Established from the lung tissue of a 2.5 month old female with cerebral anoxia, cardiac anomaly, sepsis, endocardial cushion defect and foetal alcoholic syndrome. The cells can undergo up to 19 population doublings. CCD 18Lu (90112706) and CCD-18Co (90070503) were derived from the lung and colon respectively from the same patient; this has been confirmed by STR profiling at ECACC. Ethnicity: Black.

Application

Ageing and genetic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Following resuscitation this cell line is initially slow to start.

Other Notes

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