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Product Name
3T3 Swiss Albino, 85022108
biological source
mouse embryo
growth mode
Adherent
karyotype
2n = 40; hypertriploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
Product has lost contact inhibition
Cell Line Origin
Mouse Swiss Albino embryo fibroblast
Cell Line Description
Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.
Application
Contact inhibition and virus studies: polyoma and SV40 viruses
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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