C6613
Anti-Cdc7 Kinase antibody, Mouse monoclonal
clone DCS-341, purified from hybridoma cell culture
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
DCS-341, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.4 μg/mL using total cell extract of A431 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDC7(8317)
General description
Anti-Cdc7 Kinase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the DCS-341 hybridoma produced by the fusion of mouse myeloma cells (NS2) and splenocytes from BALB/c mice immunized with full length human Cdc7 kinase. Cell division cycle 7-related protein kinase(CDC7) is an enzyme encoded by the CDC7 gene in humans. It is highly expressed in diffuse large B cell lymphoma (DLBCL). Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. CDC7 shares nearly 45% identity with yeast Cdc7 kinase in the kinase domain.
Immunogen
full-length human Cdc7 kinase.
Application
Anti-Cdc7 Kinase antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry.
Biochem/physiol Actions
Cell division cycle 7-related protein kinase (CDC7) in addition to its role in chromosome structure maintenance and repair, is also involved in meiosis and checkpoint responses.
Cell division cycle 7-related protein kinase(CDC7) is a serine-threonine essential kinase required for the initiation of DNA replication and is involved in regulation of the cell cycle. It is high expressed in diffuse large B cell lymphoma (DLBCL). Loss of Cdc7 kinase expression results in retarded growth at both cellular and whole body levels, with especially profound impairment of germ cell development. It inhibits genotoxin-induced apoptosis and increases survival in cancer cells. It may play an important role in the development and progression of colorectal cancer. Depletion of Cdc7 induces cancer cell death regardless of its p53 status. It can also interact with and phosphorylate Claspin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jung Min Kim et al.
Mutation research, 532(1-2), 29-40 (2003-12-04)
Cdc7 kinase plays an essential role in firing of replication origins by phosphorylating components of the replication complexes. Cdc7 kinase has also been implicated in S phase checkpoint signaling downstream of the ATR and Chk1 kinases. Inactivation of Cdc7 in
An Ning Cheng et al.
Cancer letters, 337(2), 218-225 (2013-05-21)
Cdc7-Dbf4 kinase (Dbf4-dependent kinase, DDK) is an essential factor of DNA replication and DNA damage response (DDR), which is associated with tumorigenesis. However, Cdc7 expression has never been associated to the outcome of oral squamous cell carcinoma (OSCC) patients, and
Increased Activity of the S Phase Kinase Cdc7 Is Associated with Poor Outcome in Diffuse Large B Cell Lymphoma (DLBCL).
Krawczyk J, et al.
Blood (2009)
Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
Sato N, et al.
The Embo Journal, 16(14), 4340-4351 (1997)
Michael D Rainey et al.
Cell cycle (Georgetown, Tex.), 12(10), 1560-1568 (2013-04-20)
Claspin is a critical mediator protein in the DNA replication checkpoint, responsible for ATR-dependent activation of the effector kinase Chk1. Cdc7, an essential kinase required for the initiation of DNA replication, can also interact with and phosphorylate Claspin. In this
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