C2970

Monoclonal Anti-Cathepsin L antibody produced in mouse

clone 33/2, ascites fluid

• Synonym(s): Anti-CATL, Anti-CTSL, Anti-MEP
• MDL number: MFCD01321805
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 33/2, monoclonal
• mol wt: antigen (human cathepsin L) 25 kDa; antigen (human procathepsin L) 42 kDa
• contains: 15 mM sodium azide
• species reactivity: mouse, mink, human, rat
• technique(s): immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1:200 using whole cell extract of a cultured mink lung cell line
• isotype: IgG1
• UniProt accession no.: P07711
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTSL1(1514) ; mouse ... Ctsl(13039) ; rat ... Ctsl1(25697)

Description

General description

Cathepsin L is a cysteine protease and is secreted by numerous transformed cells in its inactive pre-form. It is ubiquitously expressed and is localized in nucleus. Cathepsin L have substrate interacting region located in L and R domain loops.

Specificity

Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.

Immunogen

procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Biochem/physiol Actions

Cathepsin L expression is correlated with the metastatic potential of transformed cells. Cathepsin L is capable of degrading protein constituents of the extracellular matrix, this enzyme is thought to play a crucial role in tumor progression, metastasis and other disorders where the destruction of the matrix is the major cause of disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable