This is a powdered product. The potency in solution is dependent upon the concentration at which it is prepared. The minimum potency specification for this product is 240 µg/mg. The lot specific value will be reported on the Certificate of Analysis. Please see the link below to review a sample Certificate:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A9528-5G0000247193.pdf
A9528
Amphotericin B solubilized
powder, suitable for cell culture, BioXtra
Synonym(s):
Amphotericin B from Streptomyces sp., Fungizone
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About This Item
Empirical Formula (Hill Notation):
C47H73NO17
CAS Number:
Molecular Weight:
924.08
Beilstein:
78342
MDL number:
UNSPSC Code:
12352207
eCl@ss:
34010400
PubChem Substance ID:
NACRES:
NA.76
Pricing and availability is not currently available.
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Product Name
Amphotericin B solubilized, powder, γ-irradiated, BioXtra, suitable for cell culture
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
powder
technique(s)
cell culture | mammalian: suitable
antibiotic activity spectrum
fungi
yeast
Mode of action
cell membrane | interferes
storage temp.
2-8°C
SMILES string
C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
InChI
1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
InChI key
APKFDSVGJQXUKY-INPOYWNPSA-N
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General description
Chemical structure: polyene
Application
Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.
Biochem/physiol Actions
Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.
Packaging
Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone.
Components
This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.
Caution
Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.
Preparation Note
Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.
It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.
It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.
The actual solid weight of the packaged product is calculated according to the UV purity.
Kit Components Only
Product No.
Description
- Amphotericin B ~ 45 %
- Sodium deoxycholate ~ 35 %
- Sodium phosphate balance
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT RE 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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what is the potency of Amphotericin B solubilized solution?
1 answer-
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How much DSMO do I dissolve the 100mg powder in to achieve a stock concentration of 2.5mg/mL?
1 answer-
Amphotericin B is soluble in DMSO at 30-40 mg/mL. Add 100 mg powder to 40 mL DMSO in order to achieve a 2.5 mg/mL concentration.
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What is the shelf life of Product A9528, Amphotericin B solubilized?
1 answer-
Product No. A9528 has a recommended retest date of 5 years from the initial QC release date. This date can be found on the lot-specific Certificate of Analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the amount of Amphotericin B in Product No. A9528?
1 answer-
The package size is the amount of Amphotericin B present in the vial. If weighing the product, utilize the potency value on the lot specific Certificate of Analysis as a correction factor.
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What concentration should Product A9528, Amphotericin B solubilized, be used at?
1 answer-
We recommend to use Product No. A9528 at 5.6 mg solid per Liter. Cell culture tested amphotericin B solubilized preparation is approximately 45% amphotericin B, 35% sodium deoxycholate, with the balance being sodium phosphate. The amphotericin B is at least 80% amphotericin B and 3-5% amphotericin A by HPLC. The exact amount of amphotericin B in the product can be found on the lot specific Certificate of Analysis under Potency.
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