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A25120

Sigma-Aldrich

DEAE–Sephadex® A-25 chloride form

Synonym(s):

DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®

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About This Item

CAS Number:
12609-80-2
MDL number:
MFCD00132778
UNSPSC Code:
47101511
NACRES:
NA.56
Pricing and availability is not currently available.

form

powder

Quality Level

200

technique(s)

activity assay: suitable

matrix

cross-linked dextran

matrix active group

functional group

bead size

40-125 μm (dry)

pore size

~30,000 Da exclusion limit

operating pH

2-9

capacity

3-4 meq/g ion exchange capacity

compatibility

mode of use mixed weak and strong anion exchange chromatography

General description

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Application

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Features and Benefits

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Legal Information

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000413764
0000413765
0000413764
0000413765
0000411213

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In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
P H Jones
Experimental cell research, 139(2), 401-404 (1982-06-01)
H Wang et al.
Neuroscience, 46(4), 793-806 (1992-01-01)
We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified
Isolation and study of insulin activated nitric oxide synthase inhibitory protein in acute myocardial infarction subjects.
Ray, U., et al.
Journal of Thrombosis and Haemostasis, 33(3), 218-229 (2012)
Libby Morales et al.
Talanta, 74(1), 110-118 (2008-03-29)
In this work, a characterization of reagent chromophore 5-(4-sulphophenylazo)-8-aminoquinoline [SPA] by IR and 1H RMN was carried out and a pK(a) value of 3.55+/-0.03 was found as well. An 1:2 stoichiometry for the Cu(II)-SPA complex was determined at pH 9
DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for the isolation of glycolipids.
S K Kundu
Methods in enzymology, 72, 174-185 (1981-01-01)
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Questions

1–5 of 5 Questions  

  1. How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?

    1 answer

    1. After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. How should I store Product A25120, DEAE-Sephadex® A-25 chloride?

    1 answer

    1. Unused media can be stored in the container at +4 to +30 °C.  Ensure that the screw-top is fully tightened.  Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.

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  4. How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?

    1 answer

    1. After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.

      Helpful?

  5. What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?

    1 answer

    1. According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.

      Helpful?

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