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93014

Sigma-Aldrich

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium)

greener alternative

powder, light brown, ≥10 U/g

Synonym(s):

Manganese-dependent lignin peroxidase, MnP, Peroxidase, manganese

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

fungus (white-rot fungus (Phanerochaete chrysosporium))

form

powder

specific activity

≥10 U/g

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

color

light brown

greener alternative category

shipped in

wet ice

storage temp.

−20°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium) is from the peroxidase family and is used to oxidize manganese. It may be used to study wound healing.

Biochem/physiol Actions

Manganese peroxidase catalyzes the oxidation of Mn2+ to Mn3+. Mn3+ is then in complex with oxalate, which is able to oxidize lignin. Manganese peroxidase from Phanerochaete chrysosporium binds 2 calcium ions per subunit. It binds 1 heme B (iron-protoporphyrin IX) group per subunit .

Unit Definition

One unit corresponds to the amount of enzyme, which oxidizes 1 μmole Mn2+ per minute to Mn3+ at pH 4.5 and 25 °C

Physical form

Supplied as a lyophilized powder containing ~50% sodium tartrate
only partially soluble in water or buffer

Other Notes

This product is supplied as a mixture of isozymes, mol. mass (40-65 kDa).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Almasul Alfi et al.
Journal of bioscience and bioengineering, 128(3), 290-295 (2019-04-08)
Manganese peroxidase (MnP) is a fungal heme-containing enzyme which oxidizes Mn2+ to Mn3+, a diffusible and strong non-specific oxidant capable of attacking bulky phenolic substrates. Therefore, MnP is indispensable in the polymer and paper industries. Previous attempts of MnP expression
Dong-Soo Moon et al.
Applied biochemistry and biotechnology, 168(3), 542-549 (2012-07-14)
Alkylphenols are common endocrine disrupters that are produced from the degradation of widely used surfactants. Since they cause various harmful effects on aquatic life and in humans, they should be removed from the environments being contaminated. White rot fungus Irpex
Tomer M Salame et al.
Applied and environmental microbiology, 78(15), 5341-5352 (2012-05-29)
Pleurotus ostreatus (the oyster mushroom) and other white rot filamentous basidiomycetes are key players in the global carbon cycle. P. ostreatus is also a commercially important edible fungus with medicinal properties and is important for biotechnological and environmental applications. Efficient
Kannan Pakshirajan et al.
Journal of environmental management, 101, 118-123 (2012-03-13)
Coloured industry wastewaters often contain dyes and other toxic ingredients, and, therefore, pose serious threat to the receiving environment. Among the available methods the eco-friendly biological method has gained maximum attention due to its many advantages over the traditional methods.
Alexander N Kapich et al.
Enzyme and microbial technology, 49(1), 25-29 (2011-11-25)
The peroxidation of C18 unsaturated fatty acids by fungal manganese peroxidase (MnP)/Mn(II) and by chelated Mn(III) was studied with application of three different methods: by monitoring oxygen consumption, by measuring conjugated dienes and by thiobarbituric acid-reactive substances (TBARS) formation. All

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