77958
Abberior®CAGE 590, NHS-ester
ideal for single-molecule siwtching microscopy (e.g. PALM, STORM, GSDIM)
About This Item
Recommended Products
form
solid
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 590 nm; λem 600-620 nm in PBS, pH 7
storage temp.
−20°C
General description
Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE markers can be tracked in confocal and epifluorescence microscopy to analyse molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.
Key Features
- First CAGE dye in the red fluorescent range
- Ideal for PALM, STORM, GSDIM
- Well suited as 2nd STED color (after uncaging)
Absorption Maximum, λmax: 262, 325, 351 nm (non-activated, PBS, pH 7.4)
595 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 615 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 685 −715 nm
Fluorescence Quantum Yield, η: 0.80 (after photoactivation, PBS, pH 7.47)
Application
Suitability
Analysis Note
Other Notes
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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