11093037001
Roche
Chromozym t-PA
powder, 90% (enzymatic), 0.25 mM
Synonym(s):
N-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide acetate
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About This Item
UNSPSC Code:
12352204
Pricing and availability is not currently available.
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Quality Level
Assay
90% (enzymatic)
form
powder
mol wt
636.7
packaging
pkg of 20 mg
manufacturer/tradename
Roche
concentration
0.25 mM
storage temp.
20-25°C
Related Categories
General description
N-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide acetate
Application
Use Chromozym® t-PA (tissue plasminogen activator) (C24H32N8O7S x CH3COOH) as a substrate for the determination of t-PA, both in purified preparations and in cell culture supernatants. Moreover, it can be applied to evaluate the content of one-chain and two-chain t-PA.[1]
Quality
Purity: >90% (enzymatic)
Contaminant: <0.5% free 4-nitraniline
Contaminant: <0.5% free 4-nitraniline
Sequence
N-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide-acetate
Preparation Note
Working concentration: Approximately 0.25 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym t-PA (4mM) is stable for at least two weeks if stored at 2 to 8 °C.
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym t-PA (4mM) is stable for at least two weeks if stored at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Chromozym is a registered trademark of Pentapharm AG, Basel
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Lucia Cilenti et al.
The Journal of biological chemistry, 278(13), 11489-11494 (2003-01-17)
Omi/HtrA2 is a mammalian serine protease with high homology to bacterial HtrA chaperones. Omi/HtrA2 is localized in mitochondria and is released to the cytoplasm in response to apoptotic stimuli. Omi/HtrA2 induces cell death in a caspase-dependent manner by interacting with
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