Skip to Content
Merck
All Photos(1)

Documents

10476498001

Roche

Luciferase

from Photobacterium fischeri

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Enzyme Commission number:
1.14.14.3 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

bacterial (Photobacterium fischeri)

packaging

pkg of 2 mg

manufacturer/tradename

Roche

optimum pH

6.8

shipped in

wet ice

General description

Contents: Lyophilizate
Specific activity: Approximately 15 mU/mg protein at +25°C with FMN and myristine aldehyde as the substrates, and 50 mU NAD(P)H:FMN oxidoreductase per ml assay solution (based on the relative light emission in the ATP system under the formation of pyrophosphate with 1 mU luciferase from Photinus pyralis).
Alkanal reduced FMN-oxygen oxidoreductase (1-hydroxylating, luminescing)
Luciferase from bacteria exists as a heterodimer with α and β chains.

Application

Luciferase may be used:
  • to treat mammary tumor cryosections for bioluminescence imaging studies
  • as a component of luciferase reaction mixture
  • in bioluminescence assay membrane preparations of Zymomonas mobilis

Biochem/physiol Actions

Luciferase catalyzes the conversion of reduced flavin mononucleotide in the presence of oxygen and long-chain aldehyde to flavin mononucleotide, visible light and a fatty acid. It is a reporter enzyme for sensitive quantitation. The lucifearse fusion proteins have been useful in many protein based interaction studies especially in living cells.

Quality

Contaminants: <10 mU NADH:FMN oxidoreductase/mg protein, <10 mU myokinase/mg protein, and <1 mU NADH oxidase/mg protein

Storage and Stability

Store at 2 to 8 °C. (A decrease in activity of approximately 20% may occur within 12 months.)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A Eberhard et al.
Biochemistry, 20(9), 2444-2449 (1981-04-28)
Synthesis of bacterial luciferase in some strains of luminous bacteria requires a threshold concentration of an autoinducer synthesized by the bacteria and excreted into the medium. Autoinducer excreted by Photobacterium fischeri strain MJ-1 was isolated from the cell-free medium by
S Walenta et al.
International journal of radiation oncology, biology, physics, 51(3), 840-848 (2001-11-09)
It has been shown that oxygen gradients exist in R3230AC tumors grown in window chambers. The fascial surface is better oxygenated than the tumor surface. The purpose of the present study was to determine whether gradients exist for energy metabolites
Nicole Feldmann et al.
Molecular and cellular endocrinology, 338(1-2), 46-57 (2011-03-05)
Glutamate is generated during nutrient stimulation of pancreatic islets and has been proposed to act both as an intra- and extra-cellular messenger molecule. We demonstrate that glutamate is not co-secreted with the hormones from intact islets or purified α- and
Interspecific luciferase beta subunit hybrids between Vibrio harveyi, Vibrio fischeri and Photobacterium leiognathi
Almashanu S, et al.
Protein engineering, design & selection : PEDS, 9(9), 803-809 (1996)
Uldis Kalnenieks et al.
Microbiology (Reading, England), 149(Pt 7), 1739-1744 (2003-07-12)
The respiratory inhibitor cyanide stimulates growth of the ethanologenic bacterium Zymomonas mobilis, perhaps by diverting reducing equivalents from respiration to ethanol synthesis, thereby minimizing accumulation of toxic acetaldehyde. This study sought to identify cyanide-sensitive components of respiration. In aerobically grown
View All Related Papers

Articles

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service