Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Store BioTracker 655 Red Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light. Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
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Can this product be used to bind to cell membrane of human smooth muscle cells?
1 answer
Technical Support
·a year ago
This product is a lipophilic carbocyanine membrane dye, it is not species-specific. This compound is not tested specifically with human smooth muscle, however, there is no reason to suspect that this material would not be suitable. Please see the links below to review additional publications that may be helpful: Histochemistry 1992 May;97(4):329-33. doi: 10.1007/BF00270034. PMID: 1618647 https://pubmed.ncbi.nlm.nih.gov/1618647/ ACS Omega 2020, 5, 20, 11829–11835 https://doi.org/10.1021/acsomega.0c01416https://pubs.acs.org/doi/10.1021/acsomega.0c01416
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