MABE941
Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody
clone 5D10D4, from rat
Synonym(s):
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5D10D4, monoclonal
species reactivity
human
technique(s)
ChIP: suitable (ChIP-seq)
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer28)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family.
Immunogen
KLH-conjugated linear peptide corresponding to human phospho Histone H3.1 (Ser28).
Application
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to be suitable for ChIP. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.
ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.
Dot Blot Specificity Analysis: A representative lot of this antibody at a concentration of 0.5ug/mL was evaluated for cross reactivity with a panel of modified and unmodified peptides.
Immunocytochemistry: A representative lot of this antibody was reported to detect Histone H3.1 phosphorylated at serine 28 in Mitotic DM4 cells. See Yoshimi et al. (2013)
ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013).
ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.
Dot Blot Specificity Analysis: A representative lot of this antibody at a concentration of 0.5ug/mL was evaluated for cross reactivity with a panel of modified and unmodified peptides.
Immunocytochemistry: A representative lot of this antibody was reported to detect Histone H3.1 phosphorylated at serine 28 in Mitotic DM4 cells. See Yoshimi et al. (2013)
ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28).
Quality
Evaluated by Western Blotting in a nocodozole treated HeLa cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected phospho Histone H3.1 (Ser28) in a nocodozole treated HeLa cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected phospho Histone H3.1 (Ser28) in a nocodozole treated HeLa cell lysate.
Target description
~ 17 kDa observed
Linkage
Replaces: MABE13
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The ability of human pluripotent stem cells (hPSCs) to specialize in neuroepithelial tissue makes them ideal candidates for use in the disease models of neural tube defects. In this study, we cultured hPSCs in suspension with modified neural induction method
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